Table 3.

Stabilization effect of [Ni₂L₃]⁴⁺-M (M) and [Ni₂L₃]⁴⁺-P (P) on different telomeric G-quadruplex DNA in 10 mM Tris, 100 mM NaCl (or 10 mM KCl), pH 7.2 buffer

DNA sequence	In sodium buffer				In potassium buffer
	Structure	DNA Tm	DNA+M ΔTm (°C)	DNA+P ΔTm (°C)	Structure	DNA Tm	DNA+M ΔTm (°C)	DNA+P ΔTm (°C)
22mer-AG3(T2AG3)3	Human Telomeric Repeat, Antiparallel36	59.6	0	10.6	Human Telomeric Repeat, Hybrid	54.6	11.7	19.8
22mer- G4(T2G4)3	Tetrahymena Telomeric Repeat, Hybrid37	62.2	−3.1	−1.9	Tetrahymena Telomeric Repeat, Hybrid39	77.6	−4.4	−5.0
32mer-(T4G4)4	Oxytricha Telomeric Repeat, Antiparallel38	62.8	−5.0	−5.0	Oxytricha Telomeric Repeat, Antiparallel40	86.9	−6.0	−5.7
26mer AA-AG3(T2AG3)3-AA	Antiparallel	47.5	−0.8	10.2	Hybrid42	61.8	7.0	18.8
26mer TT-AG3(T2AG3)3-TT	Antiparallel	52.6	0	9.8	Hybrid42	59.8	5.2	14.1
24mer T-AG3(T2AG3)3-T	Antiparallel	54.3	−0.2	9.2	Hybrid42	66.6	5.1	13.2

Meltings of human telomeric G-quadruplex DNA with different flanking sequences on the 5′ and 3′ terminus were measured in 10 mM Tris, 100 mM NaCl (or 100 mM KCl), pH 7.2 buffer. M- or P-enantiomer was fixed at 1 µM. DNA concentration was 1 µM/strand. The bold data serves as telomeric DNA reference.