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. 2008 Aug 14;36(17):5427–5440. doi: 10.1093/nar/gkn527

Figure 8.

Figure 8.

Sox5 and Sox10 influence melanocyte-specific gene expression in B16 melanoma cells. Immunocytochemistry was carried out on B16 melanoma cells transfected with an shRNA vector specific for Sox10 (A, B, E, F, I and J), the corresponding scrambled version (C, D, G, H, K and L), an shRNA vector for Sox5 (M, N, Q, R, U and V), its corresponding scrambled version (O, P, S, T, W and X) and a combination of Sox5- and Sox10-specific shRNA vectors (Y and Z). Immunocytochemistry was performed using antibodies against Sox10 (A–D, M–P), Sox5 (E–H, Q–T) and Mitf (I–L, U–Z); and immunolabeling is shown in red. Transfected cells were visualized by GFP autofluorescence (B, D, F, H, J, L, N, P, R, T, V, X and Z) (green color). Arrowheads indicate transfected cells in which expression of endogenous proteins is lost or significantly reduced.