Table 3.

Birefringence decay times and fractional amplitudes^a for the single-bend tRNA species and linear controls; absence of magnesium

	Fraction fast decay	Fast decay time (μs)	Fraction slow decay	Slow decay time (μs)
4°Cb
Bovine mtRNASer (AGY)	0.69 (0.01)c	0.54 (<0.01)	0.29 (<0.01)	2.30 (0.02)
158 bp linear	0.12 (<0.01)	0.27 (0.01)	0.87 (<0.01)	2.87 (0.02)
170 bp linear	0.16 (<0.01)	0.31 (0.03)	0.82 (0.01)	3.39 (<0.01)
174 bp linear	0.15 (<0.01)	0.34 (0.01)	0.84 (<0.01)	3.66 (0.03)
20°C
Bovine mtRNASer (AGY)	0.72 (0.01)	0.34 (0.01)	0.27 (<0.01)	1.29 (0.12)
158 bp linear	0.08 (<0.01)	0.17 (0.01)	0.91 (<0.01)	1.69 (<0.01)
170 bp linear	0.12 (<0.01)	0.20 (<0.01)	0.87 (<0.01)	1.98 (<0.01)
174 bp linear	0.12 (<0.01)	0.23 (0.01)	0.87 (<0.01)	2.14 (<0.01)
37°C
Bovine mtRNASer (AGY)	0.75 (0.01)	0.23 (<0.01)	0.25 (0.01)	0.83 (0.03)
158 bp linear	0.07 (<0.01)	0.09 (<0.01)	0.92 (<0.01)	1.07 (<0.01)
160 bp linear	0.33 (0.03)	0.17 (<0.01)	0.67 (0.03)	0.98 (0.01)
170 bp linear	0.09 (<0.01)	0.14 (<0.01)	0.90 (<0.01)	1.28 (0.01)
174 bp linear	0.14 (0.01)	0.17 (0.01)	0.85 (0.01)	1.40 (0.01)

^aTEB measurements were performed as described in Materials and methods section. For each RNA species, the fractional decay amplitudes and times represent at least 10 TEB measurements on two independent RNA preparations.

^bPrecise temperatures are provided in Materials and methods section.

^cNumbers in parentheses represent standard error.