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. 2008 Sep 4;36(17):5635–5644. doi: 10.1093/nar/gkn557

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© 2008 The Author(s)

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 5. — Analysis of the religation rate using a suicide substrate. (A) In the oligonucleotide-based suicide substrate, cleavage of the 5′-radiolabeled scissile strand (14 nt) liberates an AG dinucleotide to trap the covalent DNA–hTop1p complex. (B) The percentage of DNA relegation, in the presence and in the absence of CPT, was determined by ImageQuant software and normalized to the total amount of radioactivity in each lane: hTop1p (filled square), htop1Thr729Pro (filled traingle), htop1Thr729Lys.