Figure 1.
(a) Stretching (solid line)–relaxation (dashed line) curves in the absence of protein (black) at a pulling rate of 250 nm/s and in the presence of 30 μM gp2.5 at pulling rates of 250 nm/s (red), 100 nm/s (green), 25 nm/s (blue) and 5 nm/s (light blue) in 10 mM Hepes (pH 7.5), 50 mM Na+ (45 mM NaCl and 5 mM NaOH). (b) Stretching (solid line)–relaxation (dashed line) curves in the absence of protein (black) at a pulling rate of 250 nm/s and in the presence of 530 nM gp2.5-Δ26C at pulling rates of 250 nm/s (red), 100 nm/s (green), 25 nm/s (blue) and 5 nm/s (light blue) in 10 mM Hepes (pH 7.5), 100 mM Na+ (95 mM NaCl and 5 mM NaOH).