Table 5.
Comparison of thermodynamic data obtained from DSC and UV study performed in 10 mM sodium cacodylate buffer, pH 7.4, 100 mM KCl
| Oligo name | Gene | DSCa |
UVb |
||||
|---|---|---|---|---|---|---|---|
| Tm (°C) | ΔHcal (kcal/mol) | ΔScal (cal/mol/K) | Tm (°C) | ΔHvH (kcal/mol) | ΔSvH (cal/mol/K) | ||
| Q1 | VEGF | 82.0 | −53.0 ± 0.4 | −149.3 ± 1.1 | 80.8 | −50.6 ± 0.5 | −142.1 ± 1.4 |
| Q2 | WNT3 | 87.5 | −105.0 ± 0.8 | −291.0 ± 2.2 | 86.0 | −101.5 ± 2.5 | −277.0 ± 5.0 |
| Q4 | PDGFB | 84.8 | −73.0 ± 0.6 | −204.0 ± 1.6 | 83.0 | −70.0 ± 1.2 | −195.0 ± 3.4 |
| Q5 | JUN B | 80.2 | −28.5 ± 1.7 | −80.7 ± 0.8 | 77.7 | −26.0 ± 0.1 | −73.5 ± 0.3 |
| Q11 | FES | 60.5 | −55.5 ± 0.4 | −166.4 ± 1.2 | 58.0 | −53.0 ± 0.5 | −160 ± 1.1 |
| Q14 | FOSL1 | 75.6 | −39.4 ± 0.5 | −113.0 ± 1.5 | 73.7 | −36.0 ± 0.2 | −101 ± 0.5 |
| Q30 | c-KIT | 73.0 | −32.0 ± 0.6 | −92.5 ± 1.7 | 71.6 | −29.0 ± 0.2 | −82.0 ± 0.4 |
| Q34 | AKT2 | 87.8 | −69.0 ± 0.8 | −191.2 ± 2.2 | 87.0 | −66.0 ± 1.8 | −182.0 ± 5.1 |
| Q58 | THPO | 88.0 | −72.0 ± 0.7 | −200.0 ± 2.0 | 87.0 | −69.0 ± 4.0 | −205.5 ± 11.0 |
aDSC study was performed at 50 µM oligonucleotide concentration. The parameters were obtained from three experimental replicas through two-state model with zero heat capacity change. Tm values differed by ±1.0°C.
bUV melting study was performed at 2 µM oligonucleotide concentration and the thermodynamic parameters were obtained from non-linear curve fitting. Tm values differed by ±1.5°C.