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. 2008 Aug 30;36(17):5571–5580. doi: 10.1093/nar/gkn538

Figure 3.

Figure 3.

Activation of SNAT2 transcription by the AAR does not require enhanced recruitment of Mediator. HepG2 cells were incubated in MEM or MEM lacking histidine for 1 h, 2 h, 4 h or 8 h and then MED1 or MED23 association with the SNAT2 promoter or AARE region was monitored by ChIP analysis (a). Immunoprecipitation with a nonspecific IgG (n/s IgG) served as a negative control. MCF-7 cells were incubated in DMEM or DMEM plus 100 nM β-estradiol (E2) for 1 h and MED1, MED23 or CDK8 association with the pS2 promoter was monitored by ChIP analysis (b). On the left side, the data are presented as the ratio of protein bound to total input DNA. On the right side, the same data are shown as the fold change induced by E2 treatment. MCF-7 cells were incubated in DMEM or DMEM lacking histidine for 2 h and MED1, MED23 or CDK8 association with the SNAT2 gene was monitored by ChIP (c).