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Reduction in Mediator expression does not block induction of SNAT2 transcription. MCF-7 cells were treated with siRNA and incubated in DMEM, DMEM plus E2 or DMEM lacking histidine as described in Figure 4 and then pS2 (a) or SNAT2 (b) transcription activity was analyzed by RT–qPCR. Asterisks denote transcription activity in the siMed condition that is significantly different (P < 0.05) from the siControl condition.
