Table 2.
Stabilization by addition of 3′ dangling nucleotide in 1 M NaCl to duplexes closed with wobble base pairs
| −ΔΔHoa (kcal/mol) | −ΔΔSoa (e.u.) | −ΔΔG37oa (kcal/mol) | −ΔΔHoa (kcal/mol) | −ΔΔSoa (e.u.) | −ΔΔG37oa (kcal/mol) | ||
|---|---|---|---|---|---|---|---|
| UA G | 2.9 6.6 | 6.5 17.8 | 0.9 1.1 | GA U | 2.6 4.9 | 6.8 13.2 | 0.5 0.8 |
| UC G | 2.8 2.4 | 6.7 6.2 | 0.7 0.4 | GC U | 4.0 0.9 | 10.2 1.2 | 0.8 0.5 |
| UG G | 6.4 7.2 | 17.5 19.3 | 1.0 1.2 | GG U | 1.7 5.5 | 4.4 15.0 | 0.4 0.8 |
| UU G | 1.5 4.8 | 3.1 13.6 | 0.5 0.6 | GU U | 2.2 2.3 | 5.4 5.4 | 0.5 0.6 |
aValues calculated as described in text. Top row, this study; bottom row predicted as described by (41).