Table 3.

Stabilization by addition of second 3′ dangling nucleotide in 1 M NaCl

ΔΔG37o (kcal/mol) values for addition of second 3′ overhanga
UGGCCGXZ
X	Z A	G	C	U
A	−0.1	−0.2	−0.1	−0.2
G	−0.4	−0.1	−0.9	−0.1
C	−0.0	−0.0	−0.1	+0.2
U	−0.2	−0.0	−0.2	−0.1
GGCGCUXZ
A	−0.6	−0.2	−0.4	−0.2
G	−0.2	−0.1	−0.4	−0.2
C	−0.1	−0.0	−0.1	+0.1
U	−0.2	−0.0	−0.1	+0.1
ΔΔHo(kcal/mol) values for addition of second 3′ overhanga
UGGCCGXZ
X	Z A	G	C	U
A	+1.2	−1.2	+0.2	−1.2
G	−2.4	+1.2	−0.4	+2.4
C	+0.4	−0.8	+0.7	+2.0
U	+0.3	+1.0	+0.1	+0.4
GGCGCUXZ
A	−3.4	−2.0	−2.4	+0.4
G	−0.4	+3.8	−1.2	+4.4
C	−0.9	+0.9	+0.2	+1.0
U	+1.6	−0.2	−0.7	−0.5
ΔΔSo (e.u.) values for addition of second 3′ overhanga
UGGCCGXZ
X	Z A	G	C	U
A	+4.4	−2.8	+0.8	−3.0
G	−6.5	+4.0	+1.5	+8.2
C	+1.7	−2.4	+2.5	+5.8
U	+1.6	+3.4	+0.8	+1.8
GGCGCUXZ
A	−8.7	−5.6	−6.2	+2.0
G	−0.9	+12.4	−2.3	+14.6
C	−2.6	+3.0	+1.1	+2.8
U	+5.5	−1.0	−2.0	−2.0

^aValues calculated as described in text. Values not in bold are less than the errors in the measurement and considered to be zero.

Values in italics are non-two-state.