Table 6.
Thermodynamic parameters for duplex formation in 1 M NaCla
| Average of curve fits |
Tm−1 versus log Ct plots |
|||||||
|---|---|---|---|---|---|---|---|---|
| Sequences | −ΔHo (kcal/mol) | −ΔSo (e.u.) | −ΔGo37 (kcal/mol) | Tmb (°C) | −ΔHo (kcal/mol) | −ΔSo (e.u.) | −ΔGo37 (kcal/mol) | Tmb (°C) |
| (AUGGCCG)2 | 46.8 ± 4.90 | 122.0 ± 15. | 9.0 ± 0.3 | 60.3 | 44.8 ± 1.8 | 116.3 ± 5.6 | 8.8 ± 0.1 | 59.9 |
| (CUGGCCG)2 | 51.1 ± 1.8 | 135.2 ± 5.6 | 9.2 ± 0.1 | 59.7 | 50.2 ± 0.7 | 132.5 ± 2.0 | 9.1 ± 0.1 | 59.7 |
| (GUGGCCG)2 | 48.0 ± 2.8 | 126.0 ± 8.5 | 9.0 ± 0.2 | 59.7 | 49.2 ± 1.7 | 129.7 ± 5.3 | 9.0 ± 0.1 | 59.5 |
| (UUGGCCG)2 | 43.4 ± 6.2 | 111.9 ± 18.8 | 8.7 ± 0.5 | 60.2 | 42.3 ± 3.8 | 109.1 ± 11.6 | 8.5 ± 0.2 | 59.0 |
| (UGGCCG)2c | 51.4 | 138.5 | 8.4 | |||||
| (AGCCGGU)2 | 55.9 ± 5.1 | 150.2 ± 15.7 | 9.3 ± 0.3 | 58.8 | 55.1 ± 4.4 | 147.8 ± 13.4 | 9.3 ± 0.3 | 58.7 |
| (CGCCGGU)2 | 57.9 ± 1.8 | 156.7 ± 21.5 | 9.3 ± 0.4 | 57.8 | 57.6 ± 4.8 | 156.0 ± 14.8 | 9.2 ± 0.3 | 57.5 |
| (GGCCGGU)2 | 57.2 ± 4.2 | 154.6 ± 3.3 | 9.2 ± 0.2 | 57.4 | 62.6 ± 2.5 | 171.4 ± 7.7 | 9.4 ± 0.1 | 56.9 |
| (UGCCGGU)2 | 53.8 ± 4.1 | 144.6 ± 12.5 | 9.0 ± 0.3 | 57.3 | 54.8 ± 3.4 | 147.7 ± 10.3 | 9.0 ± 0.2 | 57.0 |
| (GCCGGU)2c | 58.8 | 159.6 | 9.2 | |||||
aSolutions are 1.0 M NaCl, 10 mM sodium cacodylate, 0.5 mM EDTA pH 7.
bCalculated at 10−4 M oligomer concentration.
cRef. (34).