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. 2008 Aug 27;36(17):5530–5539. doi: 10.1093/nar/gkn530

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© 2008 The Author(s)

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — In vivo translation of wild-type and mutant Vg1 mRNA lacking the GAAA ribozyme sequence. Stage VI oocytes were injected with capped mRNA and incubated overnight in OR2 buffer containing [³⁵S]methionine/cysteine. Vg1 protein was immunoprecipitated from whole-cell extract prepared from 20 oocytes and analyzed by SDS–PAGE followed by autoradiography. Lane 1, oocytes injected with water only; lane 2, oocytes injected with wild-type mRNA; lane 3, oocytes injected with mRNA in which the GAAA ribozyme sequence was changed to CCCA.