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. 2008 Aug;20(8):2019–2032. doi: 10.1105/tpc.108.060582

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Copyright © 2008, American Society of Plant Biologists

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Figure 3. — DNA Gel Blot Analysis of bz-s Derivatives from bz-m2(D2) Ac6067.

PstI digests of genomic DNAs from bz-m2(D2) Ac6067 and bz-s derivatives H1.1, H1.4, H1.5, H3.3, H54.1, H56.1, H90.1, and H95.1 were blotted onto a membrane and hybridized to an stc1 probe (top) and a bz probe (see Supplemental Figure 1 online). The positions of the stc1 and bz fragments used as probes and the location of the PstI sites in the 226-kb McC bz haplotype contig (GenBank accession number AF391808) are shown below the map; the asterisks represent PstI sites known to be methylated in genomic DNA. The location of the inferred e-f transposition target site in each bz-s derivative is shown above the map. The following bands are detected by the stc1 probe: parental, 9.2 and 4.6 kb, Ac occupied and somatic excision sites, respectively; H3.3, a MTn transposition to an unmapped 9S site: 5.6 kb, MTn excision site (the transposed MTn was detected in a SacI digest: see Supplemental Figure 2 online); H54.1, a MTn transposition to stk1 exon 3: 7.2 and 2.6 kb, transposed MTn with Ac (germinal) and without Ac (somatic excision), respectively, and 5.6 kb, MTn excision site; H95.1, a MTn transposition in reverse orientation to the stk1 5′ UTR: 8.6 and 4.8 kb, MTn excision site plus transposed MTn with Ds (germinal) and without Ds (somatic excision), respectively, and 8.4 and 3.8 kb, transposed MTn with Ac (germinal) and without Ac (somatic excision), respectively; H90.1, a MTn excision: 5.6 kb, MTn excision site; H1.1, a 4.8-kb adjacent inversion: 7.4 and 2.8 kb, inversion with Ac (germinal) and without Ac (somatic excision), respectively; H1.4, a 106-kb adjacent inversion: 9.6 and 5.1 kb, inversion with Ac (germinal) and without Ac (somatic excision), respectively; H56.2, a 0.2-kb adjacent inversion on the Ac side: 12.7 kb, inversion with Ac and Ds; H1.5, an ITS rearrangement: 6.8 kb, rearrangement with Ac (the 2.3-kb Ac somatic excision product would have run out of the gel), and 3.1 kb, rearranged stc1 fragment from the middle of ITS.