Figure 7.

Sequences of Four New Ds 3′ Junctions at Dicentric Chromatid Bridges and of Their Corresponding Excision Products.

Sixteen separate PCR amplification reactions on Ds2(D2) Ac6067 embryo DNA were performed with combinations of two primers oriented away from the centromere (i.e., in the same orientation) and covering the bz genomic region from mkk1 to stc1. Nested PCR was then performed with either a bz or a Ds 3′ end primer and a second set of 16 primers, each of which was downstream of the corresponding primer in the first set. The latter primer combination would amplify the b-e junction of the bridge in Figure 5F. Nested PCR products were pooled in groups of eight, cloned into pGEM-T Easy, and sequenced.