Figure 2.

Enhanced Expression of RD19 Does Not Affect the Plant Response to the ΔPopP2 Strain.

(A) Expression analysis of the RD19 gene in three different genotypes after inoculation with the GMI1000 strain. RD19 transcript levels were determined by Q-RT-PCR from cDNAs generated from the aerial parts of three plants per genotype at 0, 5, and 12 DAI. The expression values of RD19 were normalized using the expression level of two housekeeping genes considered as internal standards. Mean expression and sd values were calculated from the results of two independent experiments (triplicate samples of three plants were taken at each time point). (a) Col-0 (RD19 RRS1-S), (b) Nd-1(RD19 RRS1-R), (c) RD19g-1 line (rd19 RRS1-R mutant complemented with RD19 genomic clone), (d) rd19 RRS1-S mutant (SALK_031088). AU, arbitrary units.

(B) Disease symptom developments were scored after root inoculation with the ΔPopP2 strain. Each plant was scored at 4, 5, 6, and 8 DAI of roots using a scale between 0 and 4: 0 = no wilting, 1 = 25%, 2 = 50%, 3 = 75%, and 4 =100% of leaves were wilted. Means and sd were calculated from scores of 30 plants per accession and mutant line. This experiment was repeated three times, and reproducible results were obtained; open triangle, Col-0 (RD19 RRS1-S); open square, Nd-1 (RD19 RRS1-R); closed triangle, SALK_031088 line (rd19 RRS1-S); closed square, F3-1 line (rd19 RRS1-R); open circle, RD19g-1 line (rd19 RRS1-R mutant complemented with RD19 genomic clone).