Figure 2.

Polypeptide Composition of Affinity-Purified PSII Complexes in States 1 and 2.

Polypeptides in PSII complexes, isolated using nickel affinity chromatography, were analyzed by SDS-PAGE stained with Coomassie blue (A) and immunoblotting using antibodies specific for each protein as labeled (B). C, isolated PSII cores; S1, PSII complexes isolated from State 1 thylakoid membranes; S2, PSII complexes isolated from State 2 thylakoid membranes. One microgram of chlorophyll was loaded in each lane.