Figure 1.

Phenotype of Wild-Type and mwp1-R Husk Leaves.

(A) to (G) Wild-type husk leaves.

(A) Wild-type ear covered by smooth husk leaves.

(B) Magnified view of the surface of a wild-type husk leaf.

(C) Transverse section through a wild-type husk leaf.

(D) Close-up view of a vascular bundle, showing a collateral distribution of adaxial xylem (pseudocolored pink) and abaxial phloem.

(E) Scanning electron micrograph of the abaxial epidermis.

(F) Scanning electron micrograph of the abaxial epidermis near the margin, showing characteristically long hairs.

(G) Scanning electron micrograph of the adaxial epidermis.

(H) to (O) mwp1-R husk leaves.

(H) mwp1-R ears are covered by rough husk leaves with outgrowths.

(I) Magnified view of the surface of a mwp1-R husk leaf.

(J) Transverse section through a mwp1-R husk leaf, showing the development of pairs of flaps flanking sectors of adaxialized epidermis on the abaxial side. Parts of the epidermis with adaxial and abaxial characteristics are indicated by blue and yellow lines, respectively.

(K) Magnified view of (J), showing that the vascular bundles in the flaps have xylem oriented toward the inner, adaxialized side.

(L) and (M) Examples of radialized bundles from mwp1-R leaf flaps, with xylem at the periphery (pseudocolored pink).

(N) Scanning electron micrograph of a piece of mwp1-R husk leaf, showing the hairy outer surface of the flaps, characteristic of abaxial margin identity.

(O) Scanning electron micrograph of the inner surface of a flap, showing adaxial sheath characteristics.

Bars = 1 mm in (B) and (I), 20 μm in (D), (L), and (M), and 200 μm in all others.