Figure 10.

The pdi5Δ Knockout Mutation Affects Seed Viability and Seed Set

(A) and (B) Tetrazolium-based embryo viability test. Bar = 500 μm.

(A) Top row: wild-type embryos show a uniform red color (viable seeds). Bottom row: wild-type embryos boiled for 30 min are light pink (negative control).

(B) Top row: pdi5Δ embryos show variable degrees of red and pink coloring (white arrowheads). Bottom row: boiled mutant embryos are light pink.

(C) and (D) Seed set in wild-type (C) and pdi5Δ mutant (D) siliques. Note the pdi5Δ mutant silique contains fewer seeds (open arrowheads) and gaps (filled arrowheads) (D) than the wild type (open arrowheads). Bars = 1 mm.