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HEK culture area versus seeding density comparison
| Culture surface | Cell seeding density (cells cm−2) (citation) |
|---|---|
| 75 cm2 flask | 1.2 × 103 (Chou et al. 2002) |
| 25 cm2 flask | 3.5 × 103 (Lonza Bioscience 2007b) |
| 6-well plate (9.6 cm2) | 1.0 × 104 (Witzmann et al. 2005) |
| 96-well plate (0.32 cm2) | 2.2 × 104 (Rouse et al. 2006) |
| Micro-channel patch (0.0009 cm2) | 0.7–1 × 105 |
