Table 2.
Recent approaches for human HSC expansion
| System | Cells | Volume (mL) | Media and growth factors | Fold expansion | Engraftment | Reference |
|---|---|---|---|---|---|---|
| RWV0–6 rpm8 days | 2 × 105 UCB-MNC/mL | 33 | IMDM, 10% FBS, 10% horse serum, 5.33 ng/mL IL-3, 16 ng/mL SCF, 3.33 ng/mL G-CSF, 2.13 ng/mL GM-CSF, 7.47 ng/mL Flt-3L and 7.47 ng/mL TPO | MNC: 435 ± 87.6CD34+: 32.7 ± 15.6CFU-GM: 21.7 ± 4.9 | Not tested | Liu et al. (2006) |
| Spinner flask30 rpm7 days | 1 × 106 Mbm CD34+/mL | 30 | IMDM, 20% FBS, 50 ng/mL SCF, 5 ng/mL IL-3 and 10 ng/mL IL-6 | MNC: 1.27CD34+: 5.43CFU: 10.60 | Not tested | Qunliang et al. (2006) |
| DIDECO Pluricell30 rpm12 days | 2 × 104 fresh UCB-CD34+/mL | 38 | Unknown with Flt-3L, TPO, IL-3, SCF and human plasma | MNC: 230.4 ± 91.5CD34+: 21.0 ± 11.9CFU: ND | Yes | Astori et al. (2005) |
| Static 3D matrixFibronectin covered14 days | 2.5 × 105 UCB-CD34+/mL | 1 | StemSpan (serum free)Cytokine free | MNC: NDCD34+: 3CFU-GM: 2.6 | Yes | Ehring et al. (2003) |
| Static 3D matrixFibronectin-immobilized PET10 days | 1.0 × 103 UCB-CD34+/mL | 0.1 | StemSpan (serum free)100 ng/mL SCF, 100 ng/mL Filt-3L, 50 ng/mLTPO, 20 ng/mL IL-3 | MNC: NDCD34+: 100LTC-IC: 47 | Yes | Feng et al. (2006) |
| Static disposable bags8 days | 1.0 × 103 UCB-CD34+/mL | 2.3–24.5 | StemSpan (serum free)100 ng/mL SCF, 100 ng/mL Flt-3L, 50 ng/mL TPO, 1 μg/mL low-density lipoproteins | MNC: 24.6 ± 3.6CD34+: 30.8 ± 7.2CFU: 31.3 ± 5.8 | Yes | Madlambayan et al. (2006) |
| Stirred tank bioreactor75 rpm7 days | 5 × 105 UCB-MNC/mL | 120 | IMDM, 10% FBS, 1 ng/mL IL-3, 5 ng/mL SCF, 1 ng/mL GM-CSF and 3 U/mL EPO | MNC: 1.27CD34+: NDCFU: 7 | Not tested | De Leon et al. (1998) |
ND: Not determined