Table 2.

Comparison of each culture medium on primary culture of hepatopancreas of Scylla serrata

Culture No.	Medium	Osmolarity (mOsm/kg)	E		F		B		R		G
			A	B	A	B	A	B	A	B	A	B
1	Leibovitz’s	485	+++	2–3	++	2–3	+	1–2	++	2–3	+++	10–15
2	2 × Leibovitz’s	740	+++	2–3	++	2–3	+	1–2	++	2–3	+++	15–20
3	3 × Leibovitz’s	950	+++	10–15	++	10	+	4–5	++	30	+++	153 days
4	Crab saline	860	+++	2–3	++	2–3	+	1–2	++	2–3	+++	10–15
5	Leibovitz’s + Crab saline(1:1)	880	+++	2–3	++	2–3	+	1–2	++	2–3	+++	184 days
6	2 × Leibovitz’s + Crab saline(1:1)	800	+++	2–3	++	2–3	+	1–2	++	2–3	+++	10–15
7	3 × Leibovitz’s + Crab saline	840	+++	2–3	++	2–3	+	1–2	++	2–3	+++	15–20
8	EDTA Citrate buffer	860	+++	5–10	++	10	+	3–5	++	30	+++	180 days
9	Leibovitz’s +Seawater	800	+++	4–6	++	5–7	+	4–5	++	10–20	+++	30–35

E, Embryonalzellen cell; F, Fibrenzellen cell; B, Blasenzellen cell; R, Restzellen cell; G, Granular cells; A, attachment of cells; B, maximum day of survival; +, approximately 30–50% of cells adhere on the culture petridish; ++, approximately 60–80% of cells adhere on the culture petridish; +++, majority of cells adheres on the culture petridish