Table 2.

2⁵⁻² Factorial experimental design for supplement screening with three replicates at the central point

Medium formulation	Independent variables					Dependent variables
	Fructose (g L−1)	Lactose (g L−1)	MET (g L−1)	TYR (g L−1)	Lipids (%)	Max. cell concentration (cells mL−1)	Viability (%)	μmax (h−1)	Max. cell Productivity (cells mL−1 h−1)
1B	1	1	2.6	0.46	3	0	0	n.d.	n.d.
2B	3	1	2.6	0.76	1	97 × 105	97	0.029	0.57 × 105
3B	1	3	2.6	0.76	1	88 × 105	99	0.017	0.30 × 105
4B	3	3	2.6	0.46	3	0	0	n.d.	n.d.
5B	1	1	2.9	0.76	3	0	0	n.d.	n.d.
6B	3	1	2.9	0.46	1	60 × 105	97	0.015	0.22 × 105
7B	1	3	2.9	0.46	1	120 × 105	97	0.018	0.40 × 105
8B	3	3	2.9	0.76	3	0	n.d.	0	n.d.
9B	2	2	2.75	0.61	2	80 × 105	91	0.015	0.19 × 105
10B	2	2	2.75	0.61	2	78 × 105	93	0.021	0.15 × 105
11B	2	2	2.75	0.61	2	79 × 105	77	0.014	0.16 × 105
TC100 + 10% FBS	n.a.	n.a.	n.a.	n.a.	n.a.	55 × 105	99	0.015	0.40 × 105
Sf900 II	n.a.	n.a.	n.a.	n.a.	n.a.	91 × 105	99	0.020	0.58 × 105

Formulations 9B to 11B correspond to the central points; MET: Methionine; TYR: Tyrosine; n.a.: Not added; n.d.: Not determined