Table 4.
Kinetic variables and parameters for S2 cell growth runs in serum-supplemented TC-100 medium, in batch mode, with different initial GLC, considering three distinct instants of cultivation: the end of exponential phase (EXP); maximum cell concentration (END); cell concentration decreasing (DEATH)
| End of exponential phase | |||||||
|---|---|---|---|---|---|---|---|
| Run | tLAG (h) | tEXP (h) | GLCEXP (g L−1) | LACEXP (g L−1) | μX,MAX (h−1) | YX/GLC (cells g−1) | |
| TC-6 | 24.0 | 120 | 7.84 | 0.059 | 0.0257 | 5.35E + 09 | |
| TC-7 | 0.0 | 96.0 | 2.88 | 0.051 | 0.0251 | 4.14E + 09 | |
| Mean | 12.0 | 108 | 5.36 | 0.055 | 0.0254 | 4.74E + 09 | |
| Standard deviation | 17.0 | 17.0 | 3.51 | 5.66E–03 | 4.26E–04 | 8.59E + 08 | |
| CV (%) | 141 | 15.7 | 65.4 | 10.3 | 1.68 | 18.1 | |
| Maximum cell growth | Beginning of death phase | |||||||
|---|---|---|---|---|---|---|---|---|
| Run | tEND (h) | GLCEND (g L−1) | LACEND (g L−1) | XV,END (cells mL−1) | PX,END (cells mL−1 h−1) | tDEATH (h) | GLCDEATH (g L−1) | LACDEATH (g L−1) |
| TC-6 | 144 | 7.53 | 0.035 | 5.50E + 06 | 3.40E + 04 | 240 | 0 | 0.05 |
| TC-7 | 96.0 | 2.88 | 0.051 | 5.40E + 06 | 5.21E + 04 | 216 | 6.10 | 0.03 |
| Mean | 120 | 5.21 | 0.043 | 5.45E + 06 | 4.31E + 04 | 228 | 3.05 | 0.04 |
| Standard deviation | 33.9 | 3.29 | 0.011 | 7.07E + 04 | 1.28E + 04 | 17.0 | 4.31 | 0.01 |
| CV (%) | 28.3 | 63.2 | 26.3 | 1.30 | 29.7 | 7.44 | 141 | 35.4 |