Table 5.
Kinetic variables and parameters for S2 cell growth runs in serum-supplemented TC-100 medium, in fed batch mode, considering three distinct instants of cultivation: the end of exponential phase (EXP); maximum cell concentration (END); cell concentration decreasing (DEATH)
| End of exponential phase | |||||||
|---|---|---|---|---|---|---|---|
| Run | tLAG (h) | tEXP (h) | GLCEXP (g L−1) | LACEXP (g L−1) | μX,MAX (h−1) | YX/GLC (cells g−1) | |
| FB-1 | 0.0 | 120 | 0.654 | 0.000 | 0.0196 | 2.46E + 09 | |
| FB-2 | 24.0 | 120 | 0.900 | 0.028 | 0.0192 | 2.72E + 09 | |
| Mean | 12.0 | 120 | 0.777 | 0.014 | 0.0194 | 2.59E + 09 | |
| Standard deviation | 17.0 | 0.0 | 0.174 | 0.020 | 0.0003 | 1.83E + 08 | |
| CV (%) | 141 | 0.0 | 22.4 | 141 | 1.31 | 7.07 | |
| Maximum cell growth | Beginning of death phase | |||||||
|---|---|---|---|---|---|---|---|---|
| Run | tEND (h) | GLCEND (g L−1) | LACEND (g L−1) | XV,END (cells mL−1) | PX,END (cells mL−1 h−1) | tDEATH (h) | GLCDEATH (g L−1) | LACDEATH (g L−1) |
| FB-1 | 145 | 0.65 | 0.000 | 5.20E + 06 | 3.24E + 04 | 241 | 1.00 | 0.0 |
| FB-2 | 144 | 0.90 | 0.016 | 3.30E + 06 | 1.94E + 04 | 264 | 0.900 | 0.0 |
| Mean | 145 | 0.78 | 0.008 | 4.25E + 06 | 2.59E + 04 | 253 | 0.950 | 0.00 |
| Standard deviation | 0.85 | 0.18 | 0.011 | 1.34E + 06 | 9.14E + 03 | 16.3 | 0.071 | 0.00 |
| CV (%) | 0.59 | 22.8 | 141 | 31.6 | 35.3 | 6.4 | 7.4 | – |