Fig. 4.

Comparison of antibody concentrations in fed-batch cultures using designed substitutes and conventional batch cultures plotted against integral of viable cells (IVC). Medium in batch cultivations with serum and with Substitute (standard concentration) were Iscove’s Modified Dulbecco’s medium (IMDM) containing 5% fetal bovine serum and IBL Media III, respectively. Fed-batch cultivations with Substitute (standard concentration) and Substitute (high concentration) were done with IMDM based initial medium containing Substitute (standard concentration) or Substitute (high concentration) instead of serum and feeding medium. The feeding medium was designed by Xie and Wang (1994)