Table 2.
Composition of culture media in the experiments
| Batch cultivation with serum (IMDM + 5% FBS) (mM) | Batch cultivation with Substitute I (IBL Media III) (mM) | Fed-batch cultivation with Substitute | ||
|---|---|---|---|---|
| Initial medium (mM) | Feeding medium (mM) | |||
| Serum or substitute for serum | 5% FBS | Substitute (standard concentration) | Substitute (standard or high concentration) | – |
| Glucose and amino acids | ||||
| Glucose | 0.75 | 17.8 | 2.0 | 115.4 |
| Alanine | – | 0.17 | – | – |
| Arginine | 0.1 | 0.74 | 0.1 | 7.4 |
| Asparagine | 0.5 | 0.22 | 0.5 | 3.08 |
| Aspartic acid | 1.0 | 0.18 | 1.0 | 8.64 |
| Cystine | 0.1 | 0.15 | – | – |
| Cysteine | – | 0.15 | 0.1 | 3.49 |
| Glutamic acid | 0.5 | 0.31 | 0.5 | 5.01 |
| Glutamine | 0.2 | 2.8 | 0.2 | 41.9 |
| Glycine | 1.0 | 0.26 | 1.0 | 5.41 |
| Histidine | 0.1 | 0.048 | 0.1 | 2.69 |
| Hydroxyproline | – | 0.038 | 0.038 | – |
| Isoleucine | 0.2 | 0.5 | 0.2 | 5.27 |
| Leucine | 0.2 | 0.52 | 0.2 | 10.1 |
| Lysine | 0.2 | 0.5 | 0.2 | 8.47 |
| Methionine | 0.1 | 0.13 | 0.1 | 2.71 |
| Phenylalanine | 0.1 | 0.23 | 0.1 | 3.98 |
| Proline | 0.2 | 0.29 | 0.2 | 2.0 |
| Serine | 0.5 | 0.3 | 0.5 | 3.19 |
| Threonine | 0.2 | 0.47 | 0.2 | 7.15 |
| Tryptophan | 0.1 | 0.047 | 0.1 | 1.37 |
| Tyrosine | 0.2 | 0.27 | 0.2 | 3.23 |
| Valine | 0.2 | 0.47 | 0.2 | 7.51 |
| B vitamine | ||||
| D-Biotin | 0.00005 | 0.002 | 0.00005 | 0.051 |
| Choline chlorede | 0.029 | 0.044 | 0.029 | 2.54 |
| Folic acid | 0.009 | 0.0057 | 0.009 | 0.09 |
| Myo-inositol | 0.04 | 0.093 | 0.04 | 1.35 |
| Niacinamide | 0.033 | 0.018 | 0.033 | 0.37 |
| Pyridoxal | 0.02 | 0.011 | 0.02 | 0.51 |
| Riboflavin | 0.001 | 0.0008 | 0.001 | 0.033 |
| Thiamine | 0.012 | 0.0068 | 0.012 | 0.084 |
| D-Ca pantothenate | 0.017 | 0.0045 | 0.017 | 0.11 |
| Vitamin B12 | 0.00005 | 0.00026 | 0.00001 | 0.007 |
| Inorganic salts | ||||
| NaHCO3 | 60.0 | 27.4 | 27.4 | – |
| HEPES | – | 18.75 | 18.75 | – |
| Ca(NO3)2 | – | 0.15 | 0.15 | – |
| KCl | 5.0 | 4.3 | 4.3 | – |
| MgSO4 | 2.0 | 0.5 | 0.5 | – |
| NaCl | 60.0 | 97.0 | 97.0 | – |
| Na2HPO4 | 3.0 | 1.66 | 1.66 | – |
| KNO3 | – | 0.00038 | 0.00038 | – |
| NaH2PO4 | – | 0.45 | 0.45 | – |
| CaSO4 | – | 0.0000026 | 0.0000026 | – |
| FeSO4 | 0.1 | 0.0013 | 0.0013 | – |
| ZnSO4 | – | 0.00074 | 0.00074 | – |
| CaCl2 | 2.0 | 0.81 | 0.81 | – |
| MgCl2 | – | 0.00015 | 0.00015 | – |