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Intracellular IgM of HB4C5 cells treated with thermine HB4C5 cells were inoculated at 1 × 106 cells/mL in ITES-ERDF medium containing 2.0 mM thermine or not, and cultured for 6 h. After cultivation, intracellular IgM was labeled by anti-human IgM antibodies conjugated with FITC. Fluorescence intensity was observed by an argon ion laser scanning confocal microscope in same condition
