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Immunoblotting analysis of cross-linked products arising from a mixture of HypA, HypB, HypC, UreE and UreG. The membrane was probed with anti-HypA antiserum. The cross-linking was carried out in the presence (+) or absence (−) of each protein, as indicated above the panel. The positions of the HypA monomer and the putative HypA–UreE complex are shown with arrows. Sizes of molecular mass standards are indicated on the left.
