. Author manuscript; available in PMC: 2008 Sep 26.

Published in final edited form as: Microbiology (Reading). 2007 May;153(Pt 5):1474–1482. doi: 10.1099/mic.0.2006/003228-0

Table 1.

Strains and plasmids used in this study

Strain or plasmid	Relevant characteristics ^*	Source or reference
E. coli
TOP10	Cloning strain	Invitrogen
BL21DE3	Host strain for two-plasmid system	Stratagene
BL21Rosetta/pLysS	Host strain for overexpressing H. pylori proteins; Cm^r	Novagen
H. pylori
43504	Parental strain	ATCC^†
hypA mutant	hypA::aphA3; Km^r	Olson et al. (2001)
hypA H2A mutant	Codon for His2 replaced by Ala within hypA	Mehta et al. (2003b)
ureE mutant	ureE::aphA3; Km^r	Voland et al. (2003)
ΔureAB mutant	ΔureAB::cat; Cm^r	This study
Plasmid
pHP8080	pACYC184 derivative with H. pylori ureABIEFGH and nixA; Cm^r	McGee et al. (1999)
pET21b	Cloning and expression vector; Ap^r	Novagen
pET-HypA	pET derivative plasmid for overexpression of HypA	Mehta et al. (2003b)
pET-HypA-H2A	pET-HypA with His2 replaced by Ala	Mehta et al. (2003b)
pET-HypB	pET derivative plasmid for overexpression of HypB	Mehta et al. (2003b)
pET-HypC	pET derivative plasmid for overexpression of HypC	This study
pET-UreE	pET derivative plasmid for overexpression of UreE	Benoit & Maier (2003)
pET-UreG	pET derivative plasmid for overexpression of UreG	Mehta et al. (2003a)
pET-HypA+UreE	pET derivative plasmid for overexpression of HypA and UreE	This study

Ap, Ampicillin; Cm, chloramphenicol; Km, kanamycin.

^†

American Type Culture Collecion, Manassas, VA, USA.