FIG 1, A-D.
Optimum epitopes for the Th cell clones, with parts A to D corresponding to clones one to four respectively. Peptides were diluted in log increments from 10 μg/ml to 0.01 μg/ml. Negative control peptides in each assay include peptide truncations shorter than the minimum epitope.
Top panel: Proliferation data, results are expressed as Net CPM, the difference between proliferation of the clone in the presence of peptide pulsed and unpulsed B-LCL. Peptides were added to irradiated B-LCL at the same time T cell clones were added.
Middle panel: IFN-γ secretion quantitated by ELISPOT assay. Fifty to one hundred clone cells were added per well. Resulting spots were multiplied by 10,000 or 20,000 to give results in SFC per million. Peptides were added to B-LCL at the same time T cell clones were added.
Bottom panel: Cytolytic activity by 51Cr release assay. Peptides and 51Cr were incubated with B-LCL overnight and washed twice the day of the assay. Spontaneous lysis was 36% for clone 2, peptide EPRGSDIAG at 01. μg/ml and 45% for clone 2, peptide EPRGSDIAGTT at 1 μg/ml.