Figure 5. Survivin induced killing of CD133+ cells in vitro in response to radiation.
(A) CD133+ enriched tumor cells were infected with either AdWT or CRAd-S-pk7, 24h prior to exposure to radiation (2Gy) followed by 24 hour growth in complete media. Cytotoxicity was measured by LDH assay. (Mean ± SD is presented, * p<0.05) Experiments were performed twice with 6 replicates per condition. (B) Viral replication was determined via E1A copy number measurement using qPCR and presented as copies per ng total DNA. Experiments were performed twice in triplicates.