Figure 5.

Staurosporine and KT5926 pretreatment prevent the rounding of endothelial cells. Confluent EA.hy926 cells were pretreated for 30 min with 50 nM staurosporine (B and E), 1 μM KT5926 (C and F), or DMSO alone (A) before the addition of 10 nM thrombin (D–F) for an additional 15 min. Staining of the actin cytoskeleton was then performed as described in MATERIALS AND METHODS. Photographs (magnification, 1000×) are representative of four independent experiments.