Figure 6.
Implication of Rho in control of the endothelial cell actin cytoskeleton. Confluent HUVEC monolayers were pretreated with 10−9 M CNF1 for 16 h (A) or 10−7 M DC3B for 50 h (D and E). Thrombin (10 nM) was added during the last 15 min of the experiment in C and E. The actin cytoskeleton was stained with FITC-phalloidin as described in MATERIALS AND METHODS. Photos (magnification, 1000×) are representative of at least four independent experiments. Arrows indicate gap formation between bordering cells.