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. 1998 Sep;9(9):2639–2653. doi: 10.1091/mbc.9.9.2639

Figure 7.

Figure 7

Figure 7

Implication of Rac and Cdc42 in thrombin-induced cell rounding. (A) EA.hy926 cells were transfected with plasmids encoding green fluorescent protein (GFP) or myc-tagged GTPase mutants, as described in MATERIALS AND METHODS. The morphology of transfected cells, treated or not for 15 min with 10 nM thrombin, was observed, and 100 positive cells were scored (flat or round) per transfection condition. Data represent the mean of two independent experiments. (B) EA.hy926 cells were transfected with pEXVRac1V12 (a and c) or pEXVRac1N17 (b and d). Thrombin was added (10 nM) to cells shown in B and D 15 min before staining. Expression of Rac mutants was detected using anti-Myc antibody, and F-actin was stained with FITC-phalloidin.