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. Author manuscript; available in PMC: 2008 Sep 26.
Published in final edited form as: Biochemistry. 2007 May 25;46(24):7163–7173. doi: 10.1021/bi700399m

FIGURE 3. Brh2 anneals complementary single strands.

FIGURE 3

100mer oligonucleotide (+) and (−) strands at 12 nM each were incubated at 30° with the indicated levels of (A) Brh2 or (B) Rad52 in the presence of 0.15 μM Picogreen as described in EXPERIMENTAL PROCEDURES. (C) Reactions as in A with or without 4 mM MgCl2 were incubated at 37°. (D) Reactions containing 100mer (+) and (−) strands at 40 nM each were incubated at 37° plus or minus RPA at a ratio of nucleotide to protein of 25 to 1. Reactions containing 100mer (+) and (−) strands at 12 nM each with (E) Brh2 at 200 nM or (F) Rad52 at 100 nM plus or minus RPA at a nucleotide to protein of 25 to 1 were incubated at 37°.