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Micrococcal nuclease (MNase) footprinting of eIF4AI and eIF4AIII complexes. Denaturing PAGE of RNAs recovered after MNase digestion of reactions containing poly(U)40, AMPPNP and indicated proteins (lanes 3–9). (Lanes 1,2) poly(U)18 and poly(U)40 hydrolysis ladders. (Right panels) Darker exposure of lanes 3 and 5 with densitometry traces.
