FIGURE 5.

Effect on cap-independent translation of N-terminal eIF4GI deletion variants. (A) Structure of eIF4GI deletion variants. (B) Lysates from C were probed with myc-tag and tubulin antibodies. (C) Twenty-four hours after transfection with pcDNA, eIF4GI-e, eIF4GI-Δ1-3, or Ct expression plasmids, HeLa cells were transfected with uncapped c-myc (black bars) or VEGF (black hatched bars) IRES reporters or capped β-globin (gray bars) or uncapped inverse c-myc IRES (gray hatched bars) reporter constructs and lysed 6 h thereafter.