Skip to main content
. 2008 Oct;14(10):2115–2124. doi: 10.1261/rna.1162708

FIGURE 2.

FIGURE 2.

Reprogramming of human cancerous Colo and PC3 cells into ES-like mirPS cells with retrovirus-mediated mir-302s transfection. (A) Structure of a mir-302s-expressing SpRNAi-RGFP transgene located in the XhoI/AflII cloning site of a cytomegalovirus (CMV)-promoter-driven pLNCX2 retroviral vector (Clontech), namely, pLNCX2-rT-SpRNAi. (B) Construct of the mir-302 pre-miRNA cluster (mir-302s), which was inserted in the intron region of the SpRNAi-RGFP transgene. (C) Selection of mirPS cells using FACS flow cytometry sorting with RGFP and Oct3/4. (D) Changes of morphologies and cell division rates in mirPS cells. The first (left) and second (right) peaks of the DNA-density flow cytometry charts represented the levels of resting G0/G1 and mitotic M-phase cell populations in the entire tested cell population, respectively. The mir-gfp miRNA shared no homology with human genes. (E) Loss of migration ability in mirPS-PC3 cells compared with its original PC3 cells. (F) Formation of EBs derived from mirPS cells and their differentiation into neuron-like primordial cells with Tuj1 and ABCA2 marker expression.