FIGURE 1.

Multiple ribonucleoprotein complexes assemble with let-7 in vitro. (A) Radiolabeled let-7, mir-84, mir-234, or lin-4 was incubated with different amounts of C. elegans cell extracts and analyzed by native gel electrophoresis. The ribonucleoprotein complexes named in this study are indicated on the left. (B) Up to 500-fold excess of unlabeled let-7(wt), let-7(mut), and lin-4 RNAs were used to compete for the formation of let-7 binding complexes. (C) Quantification of fold decrease in the complexes M1 and M2 in B. The reaction without any unlabeled RNA served as control. The bars represent the error from two independent experiments.