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. 2008 Oct;14(10):2104–2114. doi: 10.1261/rna.551208

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FIGURE 4. — Formation of the let-7 complexes is affected by alg-1 or alg-2 mutations. (A) Gel retardation of labeled let-7 miRNA by extracts prepared from wild-type (N2) or alg-1(gk214) animals. (B) Gel retardation of labeled let-7 miRNA by extracts prepared from wild-type (N2) or alg-2(ok304) animals. Arrow corresponding to lanes 5–9 indicate the appearance of an extra complex in alg-2(ok304) mutants. (C) Proteins isolated from indicated let-7 binding complexes formed in the cell extract prepared from a transgenic line of C. elegans that expresses GFP-tagged ALG-1 were separated by SDS-PAGE. The presence of GFP-tagged ALG-1 or actin proteins was analyzed by Western blot. (T) Total extract.