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The let-7 sequence is required for formation of the M1 and M2 complexes. (A) Sequence alignment of RNA oligonucleotides used in the gel retardation analysis in C. elegans extracts. (Solid boxes) Nucleotides identical to the let-7 sequence at the same positions. (B) RNA oligonucleotides listed in A were analyzed for ribonucleoprotein complex formation by native gel electrophoresis.
