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ΔSRL 50S subunits are inactive in protein synthesis, but can associate with 30S subunits. (A) Poly-U mRNA directed [14C]-Phe incorporation was measured for ribosomes containing WT (■) or ΔSRL (▼) 50S subunits. Sucrose density-gradient sedimentation of WT and ΔSRL 50S subunits in the absence (B) or presence (C) of 30S subunits. Arrows indicate the position of 30S subunits, 50S subunits, or 70S ribosomes.
