FIGURE 8.

Detailed stereo views of the ΔSRL-affected region of the 50S subunit. (A) The region of 23S rRNA indicated in dark blue in Figure 7B is shown (gray). Bases that are protected (orange) or enhanced (blue and pink) in ΔSRL 50S subunits are indicated as spheres placed at the N1 positions of A or G or the N3 of C; enhanced nucleotides that interact directly with protein L16 are shown in pink. P-tRNA (orange), A-tRNA (yellow), proteins L6 and L16 (pink), helix 95 (yellow), and the SRL (red) are indicated. (B) As in A, with tRNAs removed for clarity. (C) 23S rRNA helix numbers and domains are indicated: domain II (blue), V (purple and orange), and VI (yellow); and the SRL (red). D and E are rotated 180° around the vertical axis relative to B and C.