Figure 9.

Enzymatic reactions occurring in the course of incubation of axonemal tubulin with the Paramecium cytoplasmic extract. Times (minutes) are noted at the top of the figure. Protein amounts: 4 μg extract and 0.2 μg tubulin. (A) Kinetics of reactions and immunoreactivity with anti-tubulin PTM mAbs (AXO 49, TAP 952, or 6-11B-1), and anti-tubulin sequence mAbs (DM1B, or DM1B followed by DM1A) in the absence, or in the presence of 3 mM β-4 Gly peptide (+β4G). The migration levels of α- and β-tubulin bands are indicated on the Coomassie blue-stained gel (CB). (B) Immunoblotting analysis with 6-11B-1 and AXO 49 mAbs in the absence or in the presence of 20 mM sodium acetate (+Ac).