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. 2008 Sep 30;6(9):e233. doi: 10.1371/journal.pbio.0060233

Figure 2. HCF-1 Is a Ubiquitously Expressed Nuclear Protein.

Figure 2

(A) Gravid adults of wild-type and hcf-1(pk924);sur-5::gfp mutant worms were immunostained using an affinity-purified HCF-1 antibody. To ensure identical staining conditions, worms from both strains were processed on the same slide. The hcf-1(pk924) mutants were marked by SUR-5::GFP to distinguish them from wild-type worms. Endogenous HCF-1 was found to localize in the nucleus of most, if not all, somatic and germline cells in wild-type worms. The hcf-1(pk924) mutant worms marked by SUR-5::GFP showed only background signal. DAPI staining was used to indicate the nucleus. Photos were taken at 200× magnification.

(B) A magnified image of the head region of the wild-type worm shown in (A).