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. Author manuscript; available in PMC: 2009 Sep 26.
Published in final edited form as: Biochem Biophys Res Commun. 2008 Jul 11;374(3):419–423. doi: 10.1016/j.bbrc.2008.07.004

Scheme 2.

Scheme 2

Synthesis of biotinylated HaloLigands 1 and 2. Reagents and conditions: a) Boc2O, EtOH, 0 °C-r.t.; b) NaH, DMF, 0 °C; then 1-chloro-6-iodohexane, r.t.; c) TFA, anisole, CH2Cl2, r.t. then K2CO3, MeOH; d) 6 or 7, TBTU, HOBt, DMF, r.t; then 5; e) piperidine, DMF, r.t; f) Biotin-NHS, DIPEA, DMF.