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. Author manuscript; available in PMC: 2009 Sep 26.
Published in final edited form as: Biochem Biophys Res Commun. 2008 Jul 11;374(3):419–423. doi: 10.1016/j.bbrc.2008.07.004

Figure 2.

Figure 2

Direct labeling of a cell surface halo tag protein in living cells. COS7 cells expressing mHTP and EGFP as transfection marker are labeled with (A) or without (B) 20 nM of streptavidin coated QD conjugates with the HaloTag ligand 2 for 30 min in 37 °C. QD signal is acquired with 500 ms exposure. Shown images are overlaid frames of QD (red) and EGFP (green) fluorescence of the same region. Scale bar, 50 μm.