Fig. 3.
Hyperglycemia enhances the loss of myelin proteins by NRG1. (A) Fully myelinated co-cultures were incubated in medium with 25, 45, or 55 mM glucose for 7 days and treated with buffer or 25 ng/mL NRG1 for the final 48 h. Immunoblot analysis for P0 and β-actin was performed. (B) Fully myelinated co-cultures were maintained for 4 or 7 days in 25 or 55 mM glucose and treated for the final 2 days with NRG1. The expression of CNPase, Cav-1, and β-actin was determined by immunoblot analysis. Results are from one experiment repeated three times with similar outcomes.