Figure 2.

Movement of Golgi elements. Panel A shows two images of live cells expressing GFP-Sft2p, taken 1-min apart. Four optical sections spaced 1.5 μm apart (spanning the cell thickness) have been projected into a single image to minimize changes caused merely by objects shifting out of one focal plane. Arrows indicate differences in some, but not all, structures. Panel B shows single optical sections of a cell expressing GFP-Sed5p, taken 1.15 s apart. Arrows indicate the original and new position of one structure in this plane, which appears to undergo rapid movement. Bar, 1 μm.