Figure 4.

Dispersal of GFP-Sft2p in sft1 and sed5 mutants. Experiments were performed as in Figure 3. The dark areas in the cells at 37°C correspond to vacuoles. Note the loss and subsequent recovery of bright pixels revealed by the graphs. The slight residual punctate pattern in sft1–15 cells may be due to incomplete inactivation of Sft1p; other alleles showed less of this but also had a partial phenotype at 20°C.